中国现代医学杂志2005，Vol.15Issue(17)：2570-2572,2580,4.
HIF-1α反义寡核苷酸脂质体的制备和对视网膜血管内皮细胞HIF-1α表达的影响
Preparation of liposomes/HIF-1α ASODN complexes and its inhibiting effect on HIF-1αexpression in bovine retinal endothelial cell
邓爱军 1姜德咏1
作者信息
- 1. 中南大学湘雅二医院,眼科,湖南,长沙,410011
- 折叠
摘要
Abstract
[Objective] To study inhibition of HIF-1α expression in bovine retinal endothelial cell(BREC) by HIF-1α fully phosporothioated antisense oligodeoxynucleotide (HIF-1α ASODN). [Methods] BRECs were incubated with HIF-1α ASODN and were cultured in COCl2-induced hypoxic condition. HIF-1α expression was detected with immunohistochemistry. [Results] Phosphorothioate ODNs labeled with FAM was transfected into BRECs by Lipofectin. Six hours later, many green fluorescence spots were observed in the cell with the fluromicroscope, and the transfective effect was (93.8 ± 3.4)%. In no-ASODN group, the expression of HIF-1α was very low at 0 h. But it began to increase significantly at 1 h after hypoxia, and reached the peak at 4 h, 16 h later, it declined. While in ASODN group, the expression of HIF-1α was always at very low level. The two groups had significant difference (P ＜0.01). [ Conclusions ] HIF-1α antisense oligonucleotides can suppress HIF-1α expression of bovine retinal endothelial cell effectively. It has good prospect in resisting retinal angiogenesis treatment.关键词
反义寡核酸/缺氧诱导因子-1/视网膜血管内皮细胞Key words
antisense/oligonucleotides/bovine retinal endothelial cell分类
医药卫生引用本文复制引用
邓爱军,姜德咏..HIF-1α反义寡核苷酸脂质体的制备和对视网膜血管内皮细胞HIF-1α表达的影响[J].中国现代医学杂志,2005,15(17):2570-2572,2580,4.
