哈尔滨商业大学学报（自然科学版）2009，Vol.25Issue(3)：257-263,7.
PPAR γ激动剂诱导HT-29凋亡及周期阻滞的作用
Apoptosis and cell cycle arrest induced by peroxisome proliferator activated receptor gamma ligand rosiglitazone in human colorectal cancer cell line HT-29
李燕 1张文明 1陈晓光1
作者信息
- 1. 中国协和医科大学,中国医学科学院药物研究所,北京,100050
- 折叠
摘要
Abstract
Peroxisome proliferator-activated receptors (PPARs) are members of the nuclear receptor superfamily of transcription factors that respond to specific ligands by altering gene expression. PPARs control a variety of target genes involved in lipid homeostasis, diabetes and cancer. The aim of this study is to evaluate the effects of PPAR γ ligand Rosiglitazone (ROZ) on the apoptosis and cell cycle regulation in human colorectal cancer cell line HT-29 and its mechanism. Apoptosis and cell cycle kinietics with ROZ for 72 h were measured by FACS and PPAR γ protein expression by immunocytochemical staining. The level of Bcl-2, p21, p-ERK was detected by western blotting analysis. ROZ induced apoptosis in HT-29 cells, and cells were arrested in G1 phase. This effect was associated with a prominent decrease of the Bcl-2 expression and increase of p21 expression. ROZ increased expression of PPAR γ and also activated extracellular signal regulated kinase ERK. These results indicated that ROZ inhibited growth of HT-29 cells via the induction of apoptosis and cell cycle arrest in a PPAR γ dependent manner through activation of ERK pathway. It suggested that PPAR γ nuclear receptor might be a molecular target for treatment of colon tumors.关键词
PPARγ/罗格列酮/细胞周期阻滞/凋亡/ERKKey words
PPAR γ/rosiglitazone/cell cycle arrest/apoptosis/ERK分类
医药卫生引用本文复制引用
李燕,张文明,陈晓光..PPAR γ激动剂诱导HT-29凋亡及周期阻滞的作用[J].哈尔滨商业大学学报（自然科学版）,2009,25(3):257-263,7.
