生态学报2005，Vol.25Issue(10)：2582-2588,7.
球孢白僵菌对小猿叶甲的致病力测定
Pathogenicity of a Beauveria bassiana (Balsamo)Vuillemin isolate to daikon leaf beetle, Phaedon brassicae Baly (Coleoptera: Chrysomelinae)
摘要
Abstract
The daikon leaf beetle, Phaedon brassicae, is an important insect pest of cruciferous vegetables in South China. It istypically controlled by synthetic insecticides. A search was made for alternative control methods, and diseased daikon leaf beetles were collected and screened for potentially useful pathogens. A Beauveria bassiana isolate, SCAU-BB01D, originally derived from P. brassicae adults was found to infect all stages of the beetle under laboratory conditions. The pathogenicity of the isolate was investigated by immersing P. brassicae larvae and adults into serial dilutions of B. bassiana (105-108 conidia/ml). The highest concentration (108 conidia/ml) treatment caused an adult mortality of 84.7% on day 14, and larval mortalities of 94.0%, 96.0%, and 81.0% for instars Ⅰ, Ⅱ , Ⅲ on day 10, respectively. A time-dose-mortality model was used to analyze the bioassay data and the model fitted the data well, resulting in parameters for estimating the time and dose effects.The LC50 values of instar Ⅰ - Ⅲ and adult were 1.07 × 106, 1.63 × 105, and 8.31 × 106, 2.68 × 107 conidia/ml, respectively,on day 10 after treatment. The LC50 value on day 14 was 2.38× 106 conidia/ml for adults. The lethal time for the isolate on P.brassicae varied with the insect stage. At a concentration of 108 conidia/ml, the LT50 values of the isolate against adult and instar Ⅰ, instar Ⅱ , and instar Ⅲ larvae were 9.28, 4.29, 4.40 and 5.06 d, respectively. The results indicated the potential of the isolate for use in microbial control of P. brassicae.关键词
球孢白僵菌/小猿叶甲/致病力/生物防治Key words
Beauveria bassiana/Phaedon brassicae/pathogenicity/biological control分类
生物科学引用本文复制引用
何余容,吕利华,邝灼彬,冯夏,陈焕瑜,武亚静..球孢白僵菌对小猿叶甲的致病力测定[J].生态学报,2005,25(10):2582-2588,7.基金项目
国家自然科学基金资助项目(39930120) （39930120）
广东省科技计划资助项目(200320501)the National Natural Science Foundation of China (No. 39930120) and Sci-Tech Plan Project of Guangdong Province (No.200320501) （200320501）
