第一军医大学学报2001，Vol.21Issue(4)：258-260,3.
mRNA差异显示法筛选大肠癌相关基因
Screening of colorectal carcinoma-related gene by mRNA differential display
南清振 1高蕾 1杨希山 1肖冰 1周京旭 1张亚历 1施理1
作者信息
摘要
Abstract
Objective To isolate and clone colorectal carcinoma-related gene. Methods The total RNA was extracted from the tissues of human colorectal carcinoma and normal colorectal mucosa, and the specifically expressed cDNA fragments of colorectal carcinoma were screened by way of differential display polymerase chain reaction(DD-PCR). The cDNA fragments yielded from the above procedures were cloned into the plasmid TA, and subsequently underwent sequencing analysis and dot blot hybridization. Results Two novel cDNA fragments were obtained which were highly expressed in the colorectal carcinoma tissues but not in normal colorectal mucosa. Conclusion These 2 specifically expressed cDNA fragments isolated by DD-PCR might be the colorectal carcinoma-related oncogenes in quest.关键词
肠肿瘤/基因,肿瘤/基因表达/DNA变异分析分类
医药卫生引用本文复制引用
南清振,高蕾,杨希山,肖冰,周京旭,张亚历,施理..mRNA差异显示法筛选大肠癌相关基因[J].第一军医大学学报,2001,21(4):258-260,3.
