中华医学杂志（英文版）2008，Vol.121Issue(24)：2592-2598,7.
Cardiotrophin-1 induces intercellular adhesion molecule-1 expression by nuclear factor κB activation in human umbilical vein endothelial cells
Cardiotrophin-1 induces intercellular adhesion molecule-1 expression by nuclear factor κB activation in human umbilical vein endothelial cells
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作者信息
- 1. Department of Internal Medicine I, Friedrich-Schiller-University Jena, Erlanger Allee 101, 07740 Jena, Germany;Department of Internal Medicine I, Friedrich-Schiller-University Jena, Erlanger Allee 101, 07740 Jena, Germany;Department of Internal Medicine I, Friedrich-Schiller-University Jena, Erlanger Allee 101, 07740 Jena, Germany;Department of Internal Medicine I, Friedrich-Schiller-University Jena, Erlanger Allee 101, 07740 Jena, Germany;Department of Internal Medicine I, Friedrich-Schiller-University Jena, Erlanger Allee 101, 07740 Jena, Germany
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摘要
Abstract
Background In addition to elevated concentrations of cytokines, patients with congestive heart failure (CHF) show endothelial dysfunction and increased plasma concentrations of adhesion molecules like intercellular adhesion molecule-1 (ICAM-1). Furthermore, the concentration of cardiotrophin-1 (CT-1) - a cytokine of the interleukin-6 superfamily - is increased in CHF. We tested the hypothesis whether CT-1 is able to induce ICAM-1 in human umbilical vein endothelial cells (HUVEC). Furthermore we examined the signalling mechanisms of CT-1 mediated ICAM-1 expression. Methods Confluent layers of HUVEC were incubated with increasing concentrations of CT-1 (5 to 100 ng/ml) for different periods. ICAM-1 mRNA was determined by real-time polymerase chain reaction (PCR) and ICAM-1 surface expression by fluorescence-activated cell sorter (FACS) analysis and soluble ICAM-1 (slCAM-1) in the culture supematant by enzyme linked immunosorbent assay (ELISA). To clarify the signalling pathway of CT-1 induced ICAM-1 expression we used various inhibitors of possible signal transducing molecules, electromobility shift assay (EMSA) and Western blot analysis. Results CT-1 induced ICAM-1 mRNA (1.8i-0.8 fold increase compared to unstimulated cells after 6 hours) and protein (1.4~-0.2 fold increase compared to unstimulated cells after 48 hours) in HUVEC in a time- and concentration-dependent manner. EMSA experiments show that CT-1 causes nuclear factor (NF) KB activation. Because parthenolide could inhibit CT-1 induced ICAM-1 expression NFKB activation is required in this pathway. CT-1 did not activate extraceUular signal regulated kinases (ERK), c-Jun N-terminal kinase (JNK) and p38. Conclusion CT-1 is able to induce ICAM-1 in endothelial cells by NFKB activation. These results may explain in part elevated ICAM-1 concentrations in patients with CHF and endothelial dysfunction.关键词
cardiotrophin-1/intercellular adhesion molecule-1/nuclear factor κB/human umbilical vein endothelial cellsKey words
cardiotrophin-1/intercellular adhesion molecule-1/nuclear factor κB/human umbilical vein endothelial cells分类
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..Cardiotrophin-1 induces intercellular adhesion molecule-1 expression by nuclear factor κB activation in human umbilical vein endothelial cells[J].中华医学杂志（英文版）,2008,121(24):2592-2598,7.
