中国临床药理学与治疗学2004，Vol.9Issue(3)：261-269,9.
土贝母苷甲诱导HeLa细胞周期阻滞和凋亡
Induction of cell cycle arrest and apoptosis by tubeimoside I isolated from Bolbostemma paniculatum in HeLa cells
摘要
Abstract
AIM: To study the antitumor mechanism of tubeimoside I (TBMS1), and to investigate its effects on cell division cycle and cell death in HeLa cells. METHODS: Cell growth inhibition of TBMS1 was measured by MTT assay; the induction of cell cycle arrest and apoptosis by TBMS1 was determined by flow cytometry, light, fluorescence and electron microscopies, and gel electrophoresis of fragmented DNA. Western blotting was performed for detecting the apoptosis-related genes. RESULTS: TBMS1 displayed strong growth inhibitory effect against HeLa cells with estimated IC50 values of 35.7, 23.6, and 17.4 μmol*L-1. HeLa cells underwent G2/M arrest with exposure to TBMS1, and had typical morphologic and biochemical evidence of apoptosis. Western blot analysis of TBMS1-treated cells revealed downregulation of bcl-2, and overexpression of bax. CONCLUSION: TBMS1-induced cell cycle arrest and apoptosis may play an important role in antitumor effect of TBMS1, and TBMS1-induced apoptosis is closely related to downregulation of bcl-2 and overexpression of bax.关键词
土贝母/土贝母皂苷/土贝母苷甲/细胞周期/细胞凋亡/bcl-2/bax/HeLa细胞Key words
Bolbostemma paniculatum/tubeimoside I/cell cycle arrest/apoptosis/bcl-2/bax/HeLa cells分类
医药卫生引用本文复制引用
马润娣,于立坚,苏伟明,邵海艳,廖铭能,何冬梅,黄来珍..土贝母苷甲诱导HeLa细胞周期阻滞和凋亡[J].中国临床药理学与治疗学,2004,9(3):261-269,9.基金项目
Project supported by the Natural Science Foundation of Guangdong Province (№011809) （№011809）
