陕西科技大学学报（自然科学版）2006，Vol.24Issue(2)：26-30,5.
肝再生增强因子促进HepG2细胞增殖对细胞Na+,K+-ATP酶的影响
EFFECT ON Na+, K+-ATPASE IN PROLIFERATION OF HEPG2 CELL CAUSED BY AUGMENTER OF LIVER REGENERATION
摘要
Abstract
In order to elucidate the effect on Na+, K+-ATPase in proliferation of HepG2 cell caused by recombinant human augmenter of liver regeneration (rh-ALR). MTT assay was used to measure the effect of rh-ALR on the proliferation of HepG2 cell, and the activities of the Na+, K+-ATPase were measured by inorganic phosphorus spectrophotometer. The result showed that rh-ALR could promote the proliferation of HepG2 cell in vitro, and rh-ALR stimulated the activation of Na+, K+-ATPase on HepG2 cells by concentration-time depended method. The mechanism of the stimulation in Na+, K+-ATPase on HepG2cell caused by rh-ALR was fit for a Michaelis-Menten equation model. The rh-ALR promoted the turnover rate of Na+, K+-ATPase, the Vmax increased from 0.84±0.11 μmol·mg-1·min-1 to 1.68±0.07 μmol·mg-1·min-1(p<0.01), Km from 23.54±0.12 mM to 20.86±0.13 mM (p>0.05). The proliferation of HepG2 which caused by rh-ALR was inhibited by the Na+, K+-ATPase specific inhibitor, ouabain. This article presented that rh-ALR could stimulate the activation of Na+, K+-ATPase on HepG2 cells by concentration-time depended method. The proliferation of HepG2 cell caused by rh-ALR related with the activation of Na+, K+-ATPase. These results would be helpful in further research toward investigating the mechanisms of rh-ALR biological functions as the newly genetic engineering medicine.关键词
重组人肝再生增强因子(rh-ALR)/Na+,K+-ATPase/酶活/剂量-时间依赖Key words
recombinant human augmenter of liver regeneration (rh-ALR)/Na+, K+-ATPase/activity/concentration-time depended分类
生物科学引用本文复制引用
林莹,佟明华,孔祥平,梁世中..肝再生增强因子促进HepG2细胞增殖对细胞Na+,K+-ATP酶的影响[J].陕西科技大学学报（自然科学版）,2006,24(2):26-30,5.基金项目
广东省重点科技项目(99B40704G-47-1) （99B40704G-47-1）
