中国现代医学杂志2004，Vol.14Issue(11)：26-30,34,6.
大鼠血管性痴呆模型海马 CAI区神经元MAP-2和NF表达的研究
Expression of MAP-2 and NF in CAI region of hippocampal neurons in rat vascular dementia model
摘要
Abstract
Objective:To investigate the expression of MAP-2 and NF in CA1 region of hippocampal neurons and its relation with spatial learning and memory in vascular dementia model(VD).Methods:60Wistar rats with normal learning and memory confirmed by Morris water maze were divided into 4 groups group(GI/R).According to animal surviving period,the last three groups were subdivided into three groups,7,14 and 30days.These rats were tested again in Morris water maze before being sacrificed.Expression of MAP-2 and NF were detected by immunohistochemistry and the intensity of immunostaining was measured by MedHPIAS pathologic imaging-analysis system.Coronal slices with Nissl's staining were used to count neurons in hippocampus CAI regions.Results:Compared with groups normal,VO and BCCO/R,escape latency of GI/R group was longer following elongation of reperfusion(P＜0.01).In Hippocampus CA1 regions the number of neurons was not reduced in groups VO and BCCO/R,but decreased in GI/R groups(P＜0.01),and had significant differences among the GI/R 7,14 and 30d groups(P＜0.01).Compared with normal group,expressions of MAP-2 and NF in Hippocampus CAI region obviously reduced in BCCO/R7d group(P＜0.05),but in groups BCCO/R 14 d and 30 d their expressions had no significant change(P＞0.05).In groups GI/R the neurites with immunostaining of MAP-2 and NF were not observed,but strong staining of these two proteins was present in perikaryon of the neurons(P＜0.O5).Conclusions:The expression pattern of MAP-2 and NF indicates that neuronal plasticity is the responding to injury.The finding that MAP-2 and NF were aggregated in cytoplasm might imply their protective roles to neuron in vascular dementia.关键词
血管性痴呆,Morris水迷宫/微管相关蛋白-2/神经丝蛋白Key words
vascular dementia/global cerebral isehemia and reporfusion/morris water maze/MAP-2/NF分类
医药卫生引用本文复制引用
刘正清,马志健,李明波,蔡维君,王春旭,文晓丹..大鼠血管性痴呆模型海马 CAI区神经元MAP-2和NF表达的研究[J].中国现代医学杂志,2004,14(11):26-30,34,6.基金项目
Supported by Hunan Province Natural Science Foundation(02JJY2048) （02JJY2048）
