中国药理学与毒理学杂志2006，Vol.20Issue(1)：26-32,7.
植物雌激素白藜芦醇和根皮素对家兔离体主动脉收缩反应的影响
Effects of phytoestrogens resveratrol and phloretin on contractile response of aortic strips in rabbits
摘要
Abstract
AIM To investigate whether the relaxation characteristics of phytoestrogens resveratrol and phloretin on contractile response of aortic strips are similar to that of estrogen and the mechanisms underground. METHODS Aortic strips from rabbits were suspended in organ baths containing Krebs solution, and then isometric tension was measured. RESULTS Resveratrol and phloretin inhibited the contractile responses to norepinephrine (NE), KCl and CaCl2, shifted their concentration-response curves rightward with pD2′ values of 2.89, 3.34, 3.37 for resveratrol and 3.23, 3.52, 3.77 for phloretin respectively. Also both of them concentration-dependently relaxed KCl-precontracted aortic strip. The relaxing response of resveratrol but not of phloretin in aortic strip was significantly reduced by removal of endothelium or incubation with Nω-L-nitro-arginine and methylthioninium chloride, however both their relaxant effects were not affected by indometacin and propranolol. In Ca2+-free Krebs solution containing 0.01 mmol·L-1 EGTA, resveratrol and phloretin inhibited NE-induced contraction which was caused by Ca2+ release from intracellular store, but did not affect the contraction which was induced by Ca2+ influx. CONCLUSION Resveratrol and phloretin can induce vasorelaxations which may relate to inhibition of Ca2+ influx through potential-dependent calcium channels and Ca2+ release from intracellular stores, and the relaxing response of resveratrol is endothelium-dependent in part, but of phloretin is not endothelium-dependent.关键词
植物雌激素类/白藜芦醇/根皮素/肌,平滑,血管/主动脉,胸/血管舒张/内皮,血管Key words
phytoestrogens/resveratrol/phloretin/muscle,smooth,vascular/aorta,thoracic/vasodilation/endothelium, vascular分类
医药卫生引用本文复制引用
李红芳,汪龙德,田治峰,李伟,郑天珍..植物雌激素白藜芦醇和根皮素对家兔离体主动脉收缩反应的影响[J].中国药理学与毒理学杂志,2006,20(1):26-32,7.基金项目
中国博士后科学基金资助项目 ( 2003034442)The project supported by Post-doctoral Science Foundation of China(2003034442) （ 2003034442）
