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烟草中游离氨基酸的自动分析仪测定

张峻松 贾春晓 李炎强 毛多斌 张文叶

烟草科技Issue(8):26-32,7.
烟草科技Issue(8):26-32,7.

烟草中游离氨基酸的自动分析仪测定

Determination of Free Amino Acids in Tobacco with Automatic Analyzer

张峻松 1贾春晓 1李炎强 2毛多斌 1张文叶1

作者信息

  • 1. 郑州轻工业学院,郑州市东风路5号,450002
  • 2. 中国烟草总公司郑州烟草研究院,郑州市二七路88号,450000
  • 折叠

摘要

Abstract

The free amino acids in 18 kinds of burley tobacco and 11 brands of blended type cigarette were determined by automatic amino acid analyzer (AAAA). The working conditions of AAAA were: color reagent was ninhydrin solution (flow rate at 0.30mL/min), buffering solution was sodium citrate aqueous solution (flow rate at 0.45mL/min),and detection wavelengths were 570nm and 440 nm. The results showed that: 1) the total free amino acid in imported burley tobacco was obviously higher than that in domestic tobacco; 2) asparaginic acid and glutamic acid in imported tobacco were higher than those in domestic tobacco,while the proline content in imported tobacco was lower than that in domestic tobacco; 3) the total free amino acid in imported blended type cigarette was significantly higher than that in domestic cigarette,especially for asparaginic acid,leucine,tyrosine and proline.

关键词

烟草/氨基酸/自动分析仪

Key words

Free amino acid/Automatic analyzer/Tobacco

分类

轻工纺织

引用本文复制引用

张峻松,贾春晓,李炎强,毛多斌,张文叶..烟草中游离氨基酸的自动分析仪测定[J].烟草科技,2004,(8):26-32,7.

烟草科技

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