中国免疫学杂志2001，Vol.17Issue(5)：263-265,3.
免疫PCR方法在血清抗Sm抗体测定中的应用
Detection of anti-Sm antibodies in SLE serum by immuno-PCR
曾常茜 1晏舒 2王玉国3
作者信息
- 1. 北华大学医学院免疫教研室,
- 2. 北华大学医学院第二临床学院检验科,
- 3. 长春市中心医院检验科,
- 折叠
摘要
Abstract
Objective:To apply immuno-PCR for detection of anti-Sm antibodies.Methods: Immuno-PCR was established for anti-Sm antibodies in SLE sera using SaHIgG-DNA probe. The experimental conditions were optomized and methological identifieation indicated obvious in crease(×107 ) in sensitivity compared with that of ELISA. The positive rate of anti-Sm antibodies was 54.9% in SLE sera, and significantly higher than that of ELISA. Intra-and inter-CV were 2.3%～4.8% and 3.7%～6.9% respectively. Immuno-PCR was positively correlated with ELISA( p ＜0.001). Therefore, immuno-PCR for anti-Sm antibodies is specific,sensitive,reproducible and practical clinically.关键词
免疫PCR方法抗Sm抗体SLE分类
医药卫生引用本文复制引用
曾常茜,晏舒,王玉国..免疫PCR方法在血清抗Sm抗体测定中的应用[J].中国免疫学杂志,2001,17(5):263-265,3.
