国际眼科杂志2006，Vol.6Issue(2)：264-270,7.
鼠青光眼模型中热休克蛋白27的表达及其作用
Induction of heat shock protein in retinal ganglion cells and its role in a rat glaucoma model
摘要
Abstract
· AIM: To investigate whether heat shock protein 27 (HSP27) is induced in retinal ganglion cells (RGCs) in experimental rat glaucoma and whether the induction of HSP27 by intraocular pressure (IOP) elevation can increase serum autoantibody to HSP27 in the model.IOP elevation、Sham and normal groups by SPSS12.0. IOP was raised by electrocoagulating at least 3 episcleral veins and limbal veins on the right eye of each rat in IOP elevation group and its contralateral eye was used as controls. Immunohistochemical staining for HSP27 was performed in RGCs and retinal nerve fiber layer (RNFL) and serum immunoreactivity against HSP27 was detected by means of enzyme-linked immunosorbent assay (ELISA) in three groups.RNFL of the eyes with IOP elevation, while it was expressed weakly in untreated control eyes. Compared with sham and normal groups, serum autoantibody to HSP27 was slightly high at 1wk (P ＞0.05) and significantly increased at 2, 3, 4 and 8wk (P＜0.05) in IOP elevation group.enhanced expression of the endogenous HSP27 might play an important role in glaucomatous optic neuropathy.关键词
青光眼/视网膜神经节细胞/热休克蛋白27(HSP27)/酶联免疫吸附测定(ELISA)Key words
glaucoma/retinal ganglion cells/heat-shock protein 27 (HSP27)/enzyme-linked immunosorbent assay (ELISA)分类
医药卫生引用本文复制引用
吕红彬,袁援生,罗清礼,李燕,刘强..鼠青光眼模型中热休克蛋白27的表达及其作用[J].国际眼科杂志,2006,6(2):264-270,7.基金项目
中国四川省卫生厅科研计划(No.050074) （No.050074）
中国四川省教育厅科研基金(No.2005A074). Supported by the Education Department(No.2005A074) and Sanitation Department (No. 050074) of Sichuan Province （No.2005A074）
