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Identification of QTLs Related to Bolting in Brassica rapa ssp. pekinensis(syn. Brassica campestris ssp. pekinensis)

ZHANG Xiao-wei XIE Cong-hua WANG Xiao-wu WU Jian ZHAO Jian-jun SONG Xiao-fei LI Ying ZHANG Yan-guo XU Dong-hui SUN Ri-fei YUAN Yu-xiang

中国农业科学（英文版）2006，Vol.5Issue(4)：265-271,7.
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中国农业科学（英文版）2006，Vol.5Issue(4)：265-271,7.

Identification of QTLs Related to Bolting in Brassica rapa ssp. pekinensis(syn. Brassica campestris ssp. pekinensis)

Identification of QTLs Related to Bolting in Brassica rapa ssp. pekinensis(syn. Brassica campestris ssp. pekinensis)

ZHANG Xiao-wei 1XIE Cong-hua 2WANG Xiao-wu 3WU Jian 3ZHAO Jian-jun 4SONG Xiao-fei 3LI Ying 4ZHANG Yan-guo 3XU Dong-hui 3SUN Ri-fei 3YUAN Yu-xiang3

作者信息

  • 1. College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, P.R.China
  • 2. Institute of Biotechnology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, P.R. China
  • 3. Institute of Vegetables and Flowers, Chinese Academy of Agriculture Sciences, Beijing 100081, P.R. China
  • 4. Life Science College, Hebei Agricultural University, Baoding 071101, P.R. China
  • 折叠

摘要

Abstract

A genetic linkage map of Brassica rapa ssp. pekinensis was constructed with 186 AFLP (amplified fragment length polymorphism) markers by using a doubled-haploid (DH) population with 183 individuals. The individuals were derived from F1 which was developed by crossing a bolting resistant DH line Y-177-12 and an easy bolting DH line Y195-93a.AFLPs were generated by the use of restriction enzymes EcoR Ⅰ and Mse Ⅰ. The segregation of each marker and linkage was analyzed by using JoinMap version 3.0. Mapped markers were aligned in ten linkage groups which covered 887.8 cM with an average marker interval of 4.47 cM. Markers showing skewed segregation ratio were clustered in six LGs.Quantitative trait loci (QTL) were mapped for bolting resistance by using MAPQTL 4.0 package. Four QTLs explaining from 7.0 to 9.4% of the total variation were detected, all of them increase bolting resistance. These mapped QTLs could be used to develop a marker assisted selection programme for bolting resistance breeding.

关键词

bolting/QTL/linkage map/Chinese cabbage

Key words

bolting/QTL/linkage map/Chinese cabbage

分类

农业科技

引用本文复制引用

ZHANG Xiao-wei,XIE Cong-hua,WANG Xiao-wu,WU Jian,ZHAO Jian-jun,SONG Xiao-fei,LI Ying,ZHANG Yan-guo,XU Dong-hui,SUN Ri-fei,YUAN Yu-xiang..Identification of QTLs Related to Bolting in Brassica rapa ssp. pekinensis(syn. Brassica campestris ssp. pekinensis)[J].中国农业科学（英文版）,2006,5(4):265-271,7.

基金项目

The study was funded by the program of Strategic Scientific Alliances Between China and the Netherlands (973 International Cooperation Project, 2004CB720405) （973 International Cooperation Project, 2004CB720405）

The Opening Lab of Vegetable Genetics and Physiology of Ministry of Agriculture （）

and the Sino-Dutch Joint Lab of Horticultural Genomics Technology, P.R.China. （）

中国农业科学（英文版）

2095-3119

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