摘要
Abstract
Aim To investigate the effect of fastigial nucleus(FN) electrical stimulation on the activity of calpain during permanent focal cerebral ischemia and to clarify it neuroprotective effect.Methods Heathy male Wistar rats were randomly divided into pretended operation(PO),FN stimulation pretended operation(PO-FN),persistant ischemia(PI) and persistant ischemia-FN stimulation(PI-FN) groups.The later two groups were divided into 1,3,6,12 and 24 h groups.FN electrical stimulation and middle cerebral artery occlusion(MCAo) model of rats were established.Western blotting was used for detecting the content of Mr=150 000 FBDP (representing calpain activity) and Nissl's staining, for scoring by neuronal number and size.Results Activities of calpain were similar in PO and PO-FN groups(P >0.05) and in a low level.Activity of calpain in Pi group increased signigicantly from ishcemia 1 h[(3 670± 600) A/μ g total protein,t=6.338,P< 0.001] and increased continuously with prolonging of ischemia duration until ischemia 24 h[(9 180± 360) A/μ g total protein, t=9.221,16.632, respectively at 3,24 h,P< 0.001].In PI-FN group,activity of calpain also increased from ischemia 1 h[(2 510± 460) A/μ g total protein] and increased continuously with prolonging of ischemia duration.But increasing level was obviously lower than that in PI group at corresponding phase(t=1.875,2.134, respectively at 1,24 h,P< 0.05).Neuronal appearance and number in PO and PO-FN groups were normal and scores were both 0.In PI group,neuronal appearance and number showed mild change and scores increased from ischemia 1 h( z=2.443, P< 0.01) .Scores increased continuously with prolonging of ischemia duration and reached climax until ischemia 24 h(4.0± 0.0)(z=1.875,2.018, respectively at 1,12 h,P< 0.05).In PI-FN group,scores in each groups within ischemia 1-12 h were singficicantly lower than those in PI groups at corresponding phases(z=1.875,2.018, respectively at 1,12 h,P< 0.05).But from ischemia 24 h(3.9± 0.2),the scores showed no significant differences compared with those in PI group at corresponding phases(P >0.05).Conclusion FN electrical stimulation can inhibit activity of calpain in focal cerebral ischemia and play neuroprotective effect.关键词
小脑核/蛋白激酶类/脑缺血Key words
cerebellar nuclei/protein kinases/brain ischemia分类
医药卫生
