广西植物2004，Vol.24Issue(3)：266-269,4.
石竹细胞悬浮培养研究
Study on cell suspension culture of Dianthus chinensis
李宗艳1
作者信息
- 1. 西南林学院园林学院,云南昆明,650224
- 折叠
摘要
Abstract
In the present research,the suspension culture clones of Dianthus chinensis can keep cell multiplication and growth-rate at high speed when the secondary cycle is arranged for 7 days. Moreover,there exist lots of embryonic cells in the culture fluid that could keep growth and division fast and promote growth and differentiation of the formed big cell groups. When conducting trans-generation,it is better if volume rate between inocula and fresh culture media is 1∶2. Because the cell multiplication and cells, growth keep the fastest under this condition. It is the high dilution rate of 1∶2 or 1∶3 that will contribute to form the embryonic cells and produce the small embryonic cell groups. Provided that a mixture of coconut milk(CK)and lacotalbumin hydrolysate(LH)is added,the growth-rate of suspension clones could be greatly improved. Adding both is better than adding only one of them. The concentration of hormone plays a key role in cells, growth and division. Compared to six different formulas,formula 2(2,4-D 1.5 mg/L+NAA 0.5 mg/L+6-BA 0.5 mg/L)is the best. Formula 5 comes second (2,4-D 1.5 mg/L+NAA 0.5 mg/L+6-BA 1.0 mg/L)and formula 1(2,4-D 1.0 mg/L+NAA 0.5 mg/L+6-BA 0.5 mg/L)comes third.关键词
石竹/细胞悬浮培养/生长Key words
Dianthus chinensis/cell suspension culture/growth分类
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李宗艳..石竹细胞悬浮培养研究[J].广西植物,2004,24(3):266-269,4.
