中国林学（英文版）2007，Vol.9Issue(4)：267-271,5.
Effect of plant growth regulators on direct somatic embryogenesis in camphor tree (Cinnamomum camphora L. ) from immature zygotic embryos and embryogenic calli induction
Effect of plant growth regulators on direct somatic embryogenesis in camphor tree (Cinnamomum camphora L. ) from immature zygotic embryos and embryogenic calli induction
摘要
Abstract
A description of a successful direct somatic embryogenesis induction from immature zygotic embryos of a camphor tree (Cinnamomum camphora L. ) is presented. After a subculture of 2-3 years,embryogenic calli could be derived from primary somaticembryos. Immature zygotic embryos were cultured on a Murashige and Skoog (MS)basal medium supplemented with a range of combinations of cytokinins (BA) and auxins(2,4-D or NAA) for somatic embryo induction. Primary somatic embryos could be induced direly in almost all PGR combinations. A positive effect of 2,4-D On somatic embryo genesis from immature zygotic embryos of camphor tree was obtained. BA at appropriate concentrations (<5mg·L-1)had an effect similar to 2,4-D,whereas high concentrations (>5mg·L-1) of BA had the effect of restraining somatic embryo induction. NAA had a less positive effect on somatic embryogenesis than 2,4-D.关键词
Cinnamomum camphora L. ,somatic embryogenesis,primary somatic embryo,secondary somatic embryogenenic,embryogenic calliKey words
Cinnamomum camphora L. ,somatic embryogenesis,primary somatic embryo,secondary somatic embryogenenic,embryogenic calli分类
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DuLi,Zhou Suo,Bao Man-zhu..Effect of plant growth regulators on direct somatic embryogenesis in camphor tree (Cinnamomum camphora L. ) from immature zygotic embryos and embryogenic calli induction[J].中国林学（英文版）,2007,9(4):267-271,5.基金项目
This study was supposed by the Natural Science Foundation of Henan Province of China(0611033300). （0611033300）
