Labeling embryonic stem cells with enhanced green fluorescent protein on the hypoxanthineguanine phosphoribosyl transferase locusOA
Labeling embryonic stem cells with enhanced green fluorescent protein on the hypoxanthineguanine phosphoribosyl transferase locus
Objective To label embryonic stem (ES) cells with enhanced green fluorescent protein (EGF P) on the hypoxanthineguanine phosphoribosyl transferase (HPRT) gene locus for t he first time to provide a convenient and efficient wa…查看全部>>
Objective To label embryonic stem (ES) cells with enhanced green fluorescent protein (EGF P) on the hypoxanthineguanine phosphoribosyl transferase (HPRT) gene locus for t he first time to provide a convenient and efficient way for cell tracking and ma nipulation in the studies of transplantation and stem cell therapy.Methods Homologous fragments were obtained by polymerase chain reaction (PCR), from whic h the gene targeting vector pHPRT-EGFP was constructed…查看全部>>
滕路;孟国良;刑阳;尚克刚;王小珂;顾军
Laboratory of Genetics, College of Life Science, Peking University, Beijing 100871, ChinaLaboratory of Genetics, College of Life Science, Peking University, Beijing 100871, ChinaLaboratory of Genetics, College of Life Science, Peking University, Beijing 100871, ChinaLaboratory of Genetics, College of Life Science, Peking University, Beijing 100871, ChinaBeijing Experimental Animal Center, Beijing 100871, ChinaLaboratory of Genetics, College of Life Science, Peking University, Beijing 100871, China
医药卫生
embryonic stem cellHPRT geneenhanced green fluorescent proteinhomologous recombination
embryonic stem cellHPRT geneenhanced green fluorescent proteinhomologous recombination
《中华医学杂志（英文版）》 2003 (2)
267-272,6
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