中国动脉硬化杂志2009，Vol.17Issue(4)：269-272,4.
槟榔碱抑制氧化型低密度脂蛋白诱导的小鼠巨噬细胞炎症因子表达及其机制
Areeoline Repressed Inflammation Factor Expression of Maerophages Stimulated by Oxidized Low Density Lipoprotein and Its Mechanism
摘要
Abstract
Aim To investigate the effect of arecoline on inflammation factors expression of RAW 264.7 murine macrophages and the relation with PPARγ. RAW 264.7 murine macrophages were co-incubated with 75 mg/L oxidized low density lipoprotein and arecoline respectively for 24 h. Methods Reverse transcription-PCR was performed to de-termine the lever of the inflammation factor and PPARγ mRNA expression. Results The cell viability was decreased when culture with the arecoline 10-3 moL/L, but not in the group of arecoline 10-6 mol/L, 10-5 mol/L and 10-4 mol/L.The inflammation factor expression in macrophages has no significant difference among control group, 10-6 mol/L, 10-5 mol/L and 10-4 mol/L arecoline groups. The mRNA levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and intercellular adhesion molecules-1 (ICAM-1) was no significant changes when the macrophages was co-incubated with 75 mg/L ox-LDL and 10-6 moL/L arecoline, but they were all decreased in ox-LDL 75 mg/L+10-4 mol/L arecoline group and ox-LDL 75 mg/L+10-5 moL/L arecoline group while the expression of PPARγ mRNA was increased in ox-LDL 75 mg/L+10-5 mol/L arecoline group. Conclusion These results suggest that arecoline could inhibit inflammation fac-tor expression of macrophages, which may be related to up-regulation of PPARγ.关键词
槟榔碱/巨噬细胞/炎症因子/过氧化体增殖物激活型受体Key words
Arecoline/ Macrophages/ Inflammation Factor/ Peroxisome Proliferator-Activated Receptorγ分类
医药卫生引用本文复制引用
张伟,周寿红,凌红艳,姚起鑫,亓竹青,王光,胡弼..槟榔碱抑制氧化型低密度脂蛋白诱导的小鼠巨噬细胞炎症因子表达及其机制[J].中国动脉硬化杂志,2009,17(4):269-272,4.基金项目
湖南省科技厅项目(2006TP3016) （2006TP3016）
