南京医科大学学报（英文版）2003，Vol.17Issue(6)：270-276,7.
在兔近端小管ATP缺乏时部分肌动蛋白与非肌动蛋白相结合
The Sequestered Actin Was Bound to Non-actin Protein (s) in the ATP-depleted Rabbit Proximal Tubules
摘要
Abstract
Objective: To demonstrate whether the actin breakdownfrom actin-cytoskeleton in microvilli was bound to any non-actin protein (s) in ATP-depleted rabbit proximal tubules(PT). Methods:By using DTSSP to crosslink actin and non-actin protein (s) in insoluble fraction of ATP-depleted PT, the crosslinked protein complexes were analysed by two-dimensional electrophoresis and western blotting. Results: Analysis of the crosslinked actin complexes revealed one complex that contained actin and non-actin protein. Villin was not detected. Conclusion : The results strongly suggested that some of the sequestered actin was directly bound to non-actin protein (s) other than was co-precipitated with villin or the nuclei as hydrophobic actin homopolymers .关键词
肌动蛋白/绒毛蛋白/缺血/微绒毛/肾Key words
actin/villin/ischemia/microvilli/kidney分类
医药卫生引用本文复制引用
顾洛,杜军,戈应滨,范乐明,陈琪,陈进..在兔近端小管ATP缺乏时部分肌动蛋白与非肌动蛋白相结合[J].南京医科大学学报（英文版）,2003,17(6):270-276,7.基金项目
Supported by the National Institute of Diabetes and Digestive and Kidney Diseases Grant R15 DK56599-01 to CHEN Jin （）
