中国现代医学杂志2005，Vol.15Issue(18)：2721-2725,5.
高氧致CLD新生大鼠肺组织中MMP-8和TIMP-2表达的动态改变及其对纤维化形成作用的研究
Effects of MMP-8 and TIMP-2 expression on fibrosis in lung tissue of neonatal rats with CLD induced by hyperoxia
摘要
Abstract
[Objective] To investigate the dynamic changes and the effects of matrix metalloproteinase-8(MMP 8)and tissue inhibitors of metalloproteinase-2 (TIMP-2)on interstitial fibrosis in lung of neonatal rats with CLD in duced by hyperoxia. [Methods] Full-term newborn rats were continuously exposed to oxygen (90%～95%) or room air (21% O2) after birth within 12 hours. Using Masson and Gomori staining, the areas of collagenic fibres and reticular fibres were measured by imaging analysis; the changes of MMP-8 and TIMP-2 protein and mRNA expression were measured by immunohistochemistry and RT-PCR on day 1, 3, 7, 14 and 21 in two groups. [Results] After 7 d of oxygen exposure, collagenic fibres and reticular fibres began to deposit in interstitum, the areas were larger than those in air groups on 14 d and 21 d; The expression of MMP-8 protein and mRNA are decreased from 7 d to 21 d in hyperoxia groups; The expression of TIMP-2 protein and mRNA showed no change all the time. [Conclusion] With prolonged hyperoxia, the balance of MMP-8/TIMP-2 can not be kept, collagen breakdown is inhibited, which maybe leads to collagen deposition in lung interstitium.关键词
新生大鼠/高氧/纤维化/MMP-8/TIMP-2Key words
rat/hyperoxia/fibrosis/MMP-8/TIMP-2分类
医药卫生引用本文复制引用
刘雪雁,薛辛东..高氧致CLD新生大鼠肺组织中MMP-8和TIMP-2表达的动态改变及其对纤维化形成作用的研究[J].中国现代医学杂志,2005,15(18):2721-2725,5.基金项目
Fund from Natural Sciences foundation of Liaoning Province (No: 202071) （No: 202071）
