中国药科大学学报2005，Vol.36Issue(3)：272-275,4.
一种缺失核定位信号区的hbFGF的表达及纯化
Expression and Purification of a hbFGF Lacking Nuclear Localization Signal
摘要
Abstract
AIM:To study the mechanism of the unique export of one of human basic fibroblast growth factor (hbFGF) forms lacking the N-terminal nuclear localization signal (NLS),we high expressed and purified this hbFGF form in E.coli strain BL21(DE3).METHODS:The cDNA fragment of the hbFGF amplified by polymerase chain reaction (PCR) was cloned into the expression vector pET3c and expressed in BL21(DE3) by IPTG induction.The expressed hbFGF was purified by ionic exchange and heparin affinity chromatography from the supernatant of bacteria lysate.The mitogenic activity was measured by MTT.RESULTS:The expression level of hbFGF in E.coli was about 20% of the total cellular protein.The appreciable mitogenic activity of the purified hbFGF was comparable to that of hbFGF standard.CONCLUSION:The BL21(DE3)/ pET3c expression system could be used to high express hbFGF lacking NLS.The purified recombinant hbFGF was prepared and sufficient for further study.关键词
人碱性成纤维细胞生长因子/核定位信号/纯化Key words
Human basic fibroblast growth factor/Nuclear localization signal/Purification分类
生物科学引用本文复制引用
吴晓萍,苏志坚,郑青,吴思娴,冯雅,曲红艳,许华,李校堃..一种缺失核定位信号区的hbFGF的表达及纯化[J].中国药科大学学报,2005,36(3):272-275,4.基金项目
国家高技术研究发展计划("八六三"计划)资助项目(No.2001AA215131,2002AA2Z3318) （"八六三"计划）
广东省自然科学基金资助项目(No.010424) （No.010424）
