生命科学研究2005，Vol.9Issue(3)：272-277,6.
人肾上腺基因表达谱的建立及其功能的新认识
New Comprehension of Human Adrenal Functions by Profiling Gene Expression
摘要
Abstract
In order to understand the functions of human adrenal glands (AD), cDNA libraries of human normal adrenal were constructed. Large-scale sequencing expressed sequence tags (ESTs) and bioinformatics techniques were used. The categories of genes highly expressed in AD were gene/protein expression, followed by energy-metabolism. The first three genes highly expressed in AD were the enzymes and protein involved in the biosynthesis of steroids. It was first shown that some important genes were expressed in human adrenal,including hormones or secretory proteins, such as CRH, melanin concentration hormone (MCH), urocortin,cocaine and amphetamine regulated transcript (CART), and PACAP (pituitary adenylate cyclase activating polypeptide) were expressed in AD; many receptors, such as cytokine, neuropeptide, and neurotransmitter receptors, also existed in AD; cholesterol-metabolism-related genes, such as LDL receptor, HDL binding protein, cholesterol synthases. It suggests that the genes highly expressed in AD are closely related to the specific functions of adrenal. Besides steroids, many peptide hormones and cytokines are also expressed in AD. There is extensive cross-talking between adrenal and other systems by many important active peptides or cytokines. Furthermore, there may be a local CRH-ACTH-cortisol regulatory network in human adrenal.关键词
人肾上腺/基因表达谱/表达序列标签/生物信息学Key words
human adrenal gland/gene expression profile/expressed sequence tags(ESTs)/bioinformatics分类
医药卫生引用本文复制引用
彭永德,高国峰,顾彦杰,胡仁明,陈家论..人肾上腺基因表达谱的建立及其功能的新认识[J].生命科学研究,2005,9(3):272-277,6.基金项目
国家"863"计划项目(102-10-10-02) （102-10-10-02）
国家自然科学基金资助项目(39970370)Supported by National "863" High-Tech Project Grant (102-10-10-02) （39970370）
National Science and Technology Foundation Grant (39970370) （39970370）
