高技术通讯(英文版)2003,Vol.9Issue(2):27-30,4.
Medium Optimization of Tetraselmis sp.-1 Using Response Surface Methodology
Medium Optimization of Tetraselmis sp.-1 Using Response Surface Methodology
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作者信息
- 1. First Institute of Oceanography, SOA, Qingdao 266061, P.R.China;First Institute of Oceanography, SOA, Qingdao 266061, P.R.China;First Institute of Oceanography, SOA, Qingdao 266061, P.R.China;First Institute of Oceanography, SOA, Qingdao 266061, P.R.China;First Institute of Oceanography, SOA, Qingdao 266061, P.R.China
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摘要
Abstract
Response surface methodology (RSM) is used to optimize the medium of Tetraselmis sp.-1 which is cell fused microalgae capable of growing under mixotrophic condition. Empirical models are developed to describe the relationships between the operating variables (glucose, urea, sodium dehydrogenate phosphate, sodium chloride) and responses (cell density). Statistical analysis indicates that glucose and urea have significant effects on the microalgae cell density, but other two factors (sodium dehydrogenate phosphate, sodium chloride) have no obvious effect. The path of steepest ascent is used to approach the optimal region of medium composition. Optimal cell density (2.638 g dry weight/L) was reached when the operating conditions were glucose concentration (30.75 %), urea concentration (0.440 g/L), sodium dehydrogenate phosphate (15 mg/L) and sodium chloride (28 g/L).关键词
response surface methodology (RSM)/cell fused microalgae/medium/optimizationKey words
response surface methodology (RSM)/cell fused microalgae/medium/optimization分类
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..Medium Optimization of Tetraselmis sp.-1 Using Response Surface Methodology[J].高技术通讯(英文版),2003,9(2):27-30,4.