中国现代医学杂志2005，Vol.15Issue(18)：2731-2734,2745,5.
骨髓干细胞动员与移植治疗心肌梗死的比较
Comparison between mobilization and transplantation of bone marrow stem cells for therapy of myocardial infarction in rabbits
刘宏伟 1盖鲁粤 1张端珍 1金琴花 1董蔚1
作者信息
- 1. 解放军总医院,心内科,北京,100853
- 折叠
摘要
Abstract
[Objective] To compare bone marrow stem cells mobilization with bone marrow-derived mononuclear cells (BMCs) transplantation for the therapy of myocardial infarction (MI) in rabbits, explore more practical and effectively stem cells therapeutic strategy for MI. [Methods] The 30 rabbits were randomly divided into 3 groups, MI was induced by ligation of the left anterior descending artery. In mobilization group (M, n=10), granulocyte-colony days. On the 5th day the BMCs from 10 mL peripheral blood were labeled with bromodeoxyuridine (BrdU) for 24～48hours, then reinjected intravenously. In transplantation group (T, n=10), BMCs transplantation was performed 5～7days after MI. After being obtained from bone marrow (3～5 mL) of iliac crest and labeled with BrdU for 24～48hours, BMCs were transplanted into infracted myocardium through intramyocardial injection. Control animals (C, n=10) did not receive any treatment after MI. Echocardiography were performed for the evaluation of cardiac function 1 week and 5 weeks after MI, hemodynamic studies and histological study were performed 5 weeks after MI. [Results]LV ejection fraction of group M increased significantly, group T had no change, group C decreased 1 week and 5 weeks after MI. Group M and group T had higher LV max +dP/dt and max-dp/dt, lower LV end-diastolic pressure compared with group C 5 weeks after MI. Histological studies revealed that there were BrdU positive cells in the infarcted area of group M and group T, and the density of capillaries group M and group T in the infarcted area was significantly greater in comparison with group C. No regeneration of smooth muscle cells and cardiomyocytes were found in the infarcted area. [Conclusion] Bone marrow stem cells mobilization with G-CSF significantly improves the cardiac function for the therapy of MI through angiogenesis in the infarcted area. Transplantation of BMCs can avoid the deterioration of cardiac function, but the efficacy in. amelioration of cardiac function is limited. Bone marrow stem cells mobilization may offer a more practical and non-invasive therapeutic strategy for MI.关键词
动员/移植/骨髓干细胞/心肌梗塞/治疗Key words
mobilization/transplantation/bone marrow stem cells/myocardial infarction/therapy分类
医药卫生引用本文复制引用
刘宏伟,盖鲁粤,张端珍,金琴花,董蔚..骨髓干细胞动员与移植治疗心肌梗死的比较[J].中国现代医学杂志,2005,15(18):2731-2734,2745,5.
