中国林学(英文版)2002，Vol.4Issue(2)：27-32,6.
表达豇豆胰蛋白酶抑制剂转基因杨树的虫试试验
Test of Insect-Resistance of Transgenic Poplar with CpTI Gene
摘要
Abstract
Both non-transgenic hybrid triploid poplars [ ( Populus tomentosa × P. bolleana ) × P. tomentosa ] and transgenic ones expressing cowpea trypsin inhibitor were cut at the base of the stem to produce auxoblasts, and used as source of leaves for insect feeding trials performed on 3 major insect species of poplar, the forest tent caterpillar (Malacosoma disstria L. ), gypsy moth (Lymantria dispar L. ) and willow moth (Stilpnotia candida Staudinger) . The height and basal diameter of trees were measured by the end of that year (2000). The results indicated that the growth elements of transgenic poplars were not interfered by the incorporation of the CpTI gene.Intriguingly, the height and basal diameter of the clone TG04 were much greater than that of the control. The transgenic foliage consumed by insects induced the increase of larval mortality, and decrease of larval wet weight gain, faecal output, pupal weight and egg deposition. Among them 3 transgenic clones, TG04, TG07 and TG71received special attention for their outstanding insect resistance compared with other transgenic clones, which showed that the CpTI gene in them was expressed more actively and stably than in others.关键词
豇豆胰蛋白酶抑制剂(CpTI),毛白杨(Populus tomentosa),鳞翅目昆虫,抗虫性Key words
cowpea trypsin inhibitor/Populus tomentosa/Lepidoptera/insect resistance分类
农业科技引用本文复制引用
张谦,林善枝,张志毅,林元震..表达豇豆胰蛋白酶抑制剂转基因杨树的虫试试验[J].中国林学(英文版),2002,4(2):27-32,6.基金项目
Supported by the National Project in Transgenic Plant and Application （）
