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Analysis of Resistant Spectrum to Rice Blast in Transgenic Rice Lines Introduced Lysozyme Gene from T4 Phage

XU Ming-hui LI Cheng-yun LI Jin-bin TAN Xue-lin TIAN Wen-zhong TANG Zuo-shun

中国农业科学(英文版)2003,Vol.2Issue(3):273-279,7.
中国农业科学(英文版)2003,Vol.2Issue(3):273-279,7.

Analysis of Resistant Spectrum to Rice Blast in Transgenic Rice Lines Introduced Lysozyme Gene from T4 Phage

Analysis of Resistant Spectrum to Rice Blast in Transgenic Rice Lines Introduced Lysozyme Gene from T4 Phage

XU Ming-hui 1LI Cheng-yun 1LI Jin-bin 2TAN Xue-lin 3TIAN Wen-zhong 4TANG Zuo-shun4

作者信息

  • 1. Institute of Biotechnology, Yunnan Academy of Agricultural Sciences, Kunming 650223, P. R. China
  • 2. Institute of Plant Protection, Yunnan Academy of Agricultural Sciences, Kunming 650205, P. R. China
  • 3. Rice Research Institute, Yunnan Agricultural University, Kunming 650201 , P. R. China
  • 4. Institute of Genetics and Development Biology, Chinese Academy of Sciences, Beijing 100101 , P. R. China
  • 折叠

摘要

Abstract

The receptor cultivar Nan29 and thirty-six T5 rice lines derived from ten T0 generation transgen-ic plants harboring lysozyme gene were challenged in the greenhouse by inoculating 63 isolates belonging to 48races of Magnaporthe grisea from Yunnan Province. The transgenic rice lines exhibited resistance to morethan 72% of isolates inoculated in this experiment, and 38.1% (24 isolates) of them could infect the receptorcultivar Nan29. The results indicated that the transgenic rice lines possessed wide-spectrum resistance againstvarious rice blast races and the resistant spectrum of rice lines were different although some lines derived fromsame T0 plant. The transgenic rice lines exhibited also high resistance to leaf and neck blast in the disease fieldevaluation, but not all of resistant lines against leaf blast were resistant to neck blast.

关键词

Rice/Lysozyme gene/Rice blast/Resistant spectrum

Key words

Rice/Lysozyme gene/Rice blast/Resistant spectrum

分类

农业科技

引用本文复制引用

XU Ming-hui,LI Cheng-yun,LI Jin-bin,TAN Xue-lin,TIAN Wen-zhong,TANG Zuo-shun..Analysis of Resistant Spectrum to Rice Blast in Transgenic Rice Lines Introduced Lysozyme Gene from T4 Phage[J].中国农业科学(英文版),2003,2(3):273-279,7.

基金项目

This work was surported by the National Nat-ural Science Foundation of China (39960039)andthe Natural Science Foundation of Yunnan Prov-ince(2002C0079M), China. (39960039)

中国农业科学(英文版)

2095-3119

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