首页|期刊导航|中华医学杂志（英文版）|Pericytes are correlated with the permeability of rat corneal neovascular vessels induced by alkali burn
中华医学杂志（英文版）2007，Vol.120Issue(4)：274-279,6.
Pericytes are correlated with the permeability of rat corneal neovascular vessels induced by alkali burn
Pericytes are correlated with the permeability of rat corneal neovascular vessels induced by alkali burn
摘要
Abstract
Background Corneal neovascular leakage can lead to edema and secondary scarring. Previous studies have shown that pericytes play a key role in maturation of angiogenesis. The present studies investigate the relationship between vascular permeability and pericyte coverage of endothelial cells in rat corneal neovascular induced by alkali burns.Methods Corneal neovascular vessels induced by alkali burns was performed in Sprague-Dawley rats. Corneas were excised on 1, 2, 3, 5, 7 and 10 days after cauterization. The vascular permeability rate was measured by the Evans blue method. The microvessel pericyte coverage index (MPI) was applied to quantify the pericyte coverage through double immunofluorescent staining of frozen sections of corneas with CD31 as the endothelial and α-smooth muscle actin(α-SMA) as the pericyte markers. The correlation between permeability rate and MPI was analyzed. Pericyte coverage was confirmed ultrastructually using transmission electron microscopy.Results The vascular permeability rate was (1.14±0.17), (0.24±0.08), (0.29±0.16), (0.14±0.10), (0.09±0.06) and (0.05±0.04) μg·ml-1·mm-2 respectively on 1, 2, 3, 5, 7 and 10 days after cauterization. The MPI was 0, 16.07%,11.95%, 43.84%, 73.97% and 86.21% respectively at the above mentioned time points. The correlation coefficient between MPI and the permeability rate was -0.943 (P=0.005).Conclusions Pericyte recruitment was significantly correlated with the permeability of corneal neovascularization induced by alkali burns in rats. Therapeutic strategies aiming at anti-leakage should be most effective if they promote pericytes proliferation in the course of corneal neovascularization.关键词
cornea/ pericyte/ permeability/ angiogenesisKey words
cornea/ pericyte/ permeability/ angiogenesis分类
医药卫生引用本文复制引用
ZHAO Wei,JIANG Ai-hua,LI Chao-yang,YANG Wen-zhao,XU Chuan-chao,LIU Zu-guo..Pericytes are correlated with the permeability of rat corneal neovascular vessels induced by alkali burn[J].中华医学杂志（英文版）,2007,120(4):274-279,6.基金项目
This work was supported by grants from National Science Fund for Distinguished Young Scholars (No. 30225044), Fund for Innovative Research Groups of China (No. 30321004) and Natural Science Foundation of Guangdong Province of China (No. 36652). （No. 30225044）
