南京医科大学学报（英文版）2004，Vol.18Issue(5)：275-277,3.
真核表达载体pcDNA3-FHIT的构建及其在COS-1细胞中瞬时表达
Construction of Eukaryotic Expression Plasmid pcDNA3-FHIT and Its Transient Expression in COS-1 Cells
田中伟 1冯捷 1宋向凤 2彭振辉1
作者信息
- 1. 西安交通大学第二医院皮肤科,西安,710004,中国
- 2. 新乡医学院微生物学和免疫学教研室,新乡,453003,中国
- 折叠
摘要
Abstract
Objective: To construct eukaryotic expression plasmid pcDNA3-fragile histidine triad(FHIT)and obtain its transient expression in COS-1 cells. Methods: FHIT gene was cloned from normal human thyroid tissue by RT-PCR and then inserted into eukaryotic expression vector pcDNA3. After the sequence was confirmed, the recombinant plasmid pcDNA3-FHIT was transfected into COS-1 cells by cation liposome. The transient expression in the cells was measured by immunocytochemistry. Results: The sequence of FHIT in pcDNA3 was correct and high expression was obtained in COS-1 cells. Conclusion: The eukaryotic expression plasmid pcDNA3-FHIT was constructed successfully and could highly express FHIT protein in COS-1 cells. This will be potentially useful for the research on gene therapy.关键词
FHIT/基因克隆/COS-1细胞/瞬时表达Key words
fragile histidine triad/gene cloning/COS-1 cell/transient expression分类
医药卫生引用本文复制引用
田中伟,冯捷,宋向凤,彭振辉..真核表达载体pcDNA3-FHIT的构建及其在COS-1细胞中瞬时表达[J].南京医科大学学报（英文版）,2004,18(5):275-277,3.
