棉花学报2009，Vol.21Issue(4)：275-278,4.
一个陆地棉类烯醇酶基因的克隆及其表达分析
Cloning and Expressing of Putative Enolase Gene from Cotton (Gossypium hirsutum L)
摘要
Abstract
The total RNA of Gossypium hirsutum was extracted by modified CTAB method and the open reading frame of enolase gene from Gossypium hirsutum was cloned by RT-PCR,named GhEno , which was 1338 bp long and encoded 445 amino acids.The GhEno gene was registered in GenBank with the access number of EU169604.Sequence analysis alignment indicated that GhEno gene had high homology with those of other plants.The phylogenetic tree analysis showed that the GhEno was clustered in the same group with Hevea brasiliensis and Ricinus communis .Semi-quantity RT-PCR analysis indicated that expression of GhEno was detected in roots, stems and leaves,however it was stronger in roots than in leaves and stems.关键词
陆地棉/烯醇酶/基因克隆/基因表达Key words
cotton(Gossypium hirsutum L)/enolase/gene cloning/gene expression分类
农业科技引用本文复制引用
王亦学,孙毅,田颖川,吴家和..一个陆地棉类烯醇酶基因的克隆及其表达分析[J].棉花学报,2009,21(4):275-278,4.基金项目
山西省科技攻关项目(042005)资助 （042005）
