作物学报2005，Vol.31Issue(3)：275-282,8.
甘蓝型油菜遗传图谱的构建及芥酸含量的QTL分析
Construction of a Molecular Marker Linkage Map and Its Use for QTL Analysis of Erucic Acid Content in Brassica napus L.
摘要
Abstract
Erucic acid is not easily absorbed and therefore is nutritionally undesirable as the long carbon-chain fatty acid in the edible oil derived from Brassica napus. Hence, decreasing erucic acid content is one of the important objectives in double-low (low in erucic acid and low in glucosinolates) breeding program. A monogene controlling erucic acid was found in a doubled haploid (DH) population derived from a cross between a Canadian cultivar Quantum (yellow flower and low erucic acid content) and a resynthesized B. napus line No.2127-17 (white flower and high erucic acid content). In order to identify molecular markers tagging the gene controlling erucic acid, a B. napus linkage map was constructed using the 121 DH lines of the above DH population. A total of 207 markers were detected that comprised 102 random amplified polymorphic DNA (RAPD) markers, 103 simple sequence repeat (SSR) markers and one morphological marker, i.e. the flower colour and erucic acid. One hundred eighty-eight markers were assembled into 19 main linkage groups (LG1-LG19) and a minor group (A). No linkage was found between the remaining 19 markers and any of the established linkage groups. The total map length is 1 183.3 cM with an average distance of 6.3 cM between adjacent markers. The marker loci with distorted segregation (P＜0.01) accounted for 20.8% and tended to cluster in six linkage groups. The major gene for erucic acid was mapped on LG13, flanked by a co-dominant SSR marker TPS039 and a dominant RAPD marker BS164a at genetic distances of 2.2 cM and 17.1 cM, respectively. When QTL mapping was performed using the method of interval mapping, only the major gene (major QTL) was detected. The major QTL explained about 82% of the total phenotypic variation. Compared with a B. napus linkage map constructed based on a cross between N-0-9 and SYN1 in Brassica DB (http://www.ukcrop.net) using some common SSR markers, it was revealed that chromosomal rearrangements might have occurred on LG13.关键词
甘蓝型油菜/芥酸含量/分子标记/QTLKey words
Brassica napus/Erucic acid content/Molecular markers/QTL分类
农业科技引用本文复制引用
刘雪平,涂金星,刘志文,陈宝元,傅廷栋..甘蓝型油菜遗传图谱的构建及芥酸含量的QTL分析[J].作物学报,2005,31(3):275-282,8.基金项目
Supported by 973 program-the molecular biological bases of crop heterosis theory and application (973 2001CB1088). （973 2001CB1088）
