首页|期刊导航|南京医科大学学报（英文版）|Selection of DMA aptamer that specific binding human carcinoembryonic antigen in vitro
南京医科大学学报（英文版）2007，Vol.21Issue(5)：277-281,5.
Selection of DMA aptamer that specific binding human carcinoembryonic antigen in vitro
Selection of DMA aptamer that specific binding human carcinoembryonic antigen in vitro
摘要
Abstract
Objective:To select the specific aptamer of carcinoembryonic antigen (CEA), one of the most attractive molecule for cancer target therapy and imaging. Methods: Seven rounds in vitro selection were performed against the purified CEA protein. Ligand-mediated target purification and Co-immunoprecipitation were adopted to verify the specific binding of the aptamer to the purified and native protein separately. Results:The CEA-specific aptamer which can bind both the purified and native protein with the high specificity was obtained. Conclusion:This is the first time the CEA specific apatmer was produced. The results in this study provides the preliminary evidence for further investigation and application of CEA-aptamer in the future.关键词
aptamer/ carcinoembryonic antigen/ tumor targetingKey words
aptamer/ carcinoembryonic antigen/ tumor targeting分类
医药卫生引用本文复制引用
..Selection of DMA aptamer that specific binding human carcinoembryonic antigen in vitro[J].南京医科大学学报（英文版）,2007,21(5):277-281,5.基金项目
This work was supported by the China National Natural Science Foundation(30670958). （30670958）
