癌症2001，Vol.20Issue(3)：279-281,3.
8-Br-cAMP上调舌癌细胞株间隙连接蛋白基因Cx43表达的研究
Up-Regulation Effect of 8-Br-cAMP on Expression of Connexin Gene Cx43 in Tongue Carcinoma Cell Line
王安训 1黄洪章 1孔庆瑜1
作者信息
- 1. 中山医科大学孙逸仙纪念医院口腔颌面外科,
- 折叠
摘要
Abstract
Objectives：This study was designed to investigate the regulation effect of 8-Br-cAMP （ 8-Bromo 3′ ：5′ -cyclic adenosine monophosphate） on the expression of connexin gene (Cx43) in human tongue carcinoma cell line (Tca8113) . Methods：The inhibitory effect of 8-Br-cAMP on growth of Tca8113 cell line was examined by methyl thiazolyl tetrazolium (MTT) assay. Cx43 gene expression in Tca8113 cells after treated with 8-Br-cAMP was analyzed by immunocytochemistry and flow cytometry . Results：After treated with 8-Br-cAMP(10-5 mol/L, 10-4 mol/L, 10-3 mol/L), Tca8113 cells was inhibited and the inhibition rate was increased with the concentration of 8-Br-cAMP; Immunocytochemistry showed Cx43 protein detectability and up-regulation in 8-Br-cAMP treated cells; The positive rate of Cx43 protein in Tca8113 ce1ls increased from 4.8％ to 26.5％ . There was significant difference between 8-Br-cAMP treated cells and untreated cells （ P<0.01） . Conclusion： The anti-tumor effect of 8-Br-cAMP on Tca8113 cells might be due to up-regu1ation on Cx43 gene.关键词
8-Br-cAMP/间隙连接蛋白/舌癌分类
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王安训,黄洪章,孔庆瑜..8-Br-cAMP上调舌癌细胞株间隙连接蛋白基因Cx43表达的研究[J].癌症,2001,20(3):279-281,3.
