棉花学报2009，Vol.21Issue(4)：279-283,5.
棉花纤维长度主效QTLs的分子标记辅助选择及聚合效果研究
Molecular Marker-assisted Selection and Pyramiding Breeding of Major QTLs for Cotton Fiber Length
摘要
Abstract
TG41 and sGK156, two commercial cotton cultivars (lines), and HS427-10, 7235 and 0-153, three elite fiber quality germplasm lines, were used as parents to develop three double-cross combinations, (sGK156×HS427-10)×(0-153×7235), (TG41×HS427-10)×(0-153×7235) and (sGK156×0-153)×(sGK156×HS427-10).Three SSR markers, NAU1043, MUSS497 and CM67, which were linked with QTLs of fiber length and derived from 7235 or 0-153, were used to study the effect of molecular marker-assisted selection and pyramiding breeding in the three combinations, respectively.As a result, the fiber length showed significant differences between plants with and without marker in the three combinations.This indicated that the effects of the three QTLs for fiber length were stable in different genetic backgrounds and different generations.When selected the plants with two or three markers, the fiber length of plants with two or three markers were better than that of the plants with only one marker, and the fiber length was the best in the plants with all the three markers.In conclusion, it is effective to increase fiber length through molecular marker-assisted selection and the effect may be increased when more QTLs are pyramided in plants.It is necessary to develop stable recombinant inbred lines of advanced generations which pyramid several fiber-related genes to study the effect for pyramiding more genes.关键词
棉花/纤维长度/QTL/分子标记/育种Key words
cotton/fiber length/QTL/molecular marker/breeding分类
农业科技引用本文复制引用
董章辉,石玉真,张建宏,王淑芳,李俊文,刘爱英,唐淑荣,褚平,袁有禄..棉花纤维长度主效QTLs的分子标记辅助选择及聚合效果研究[J].棉花学报,2009,21(4):279-283,5.基金项目
国家973项目(2004CB117306),863计划(2006AA100105550) （2004CB117306）
