中德临床肿瘤学杂志（英文版）2008，Vol.7Issue(5)：280-282,3.
MAT1-siRNA体外转染抑制胰腺癌的细胞增殖和侵袭能力的研究
Gene silencing of MAT1 mediated by siRNA inhibits cell proliferation and invasion in pancreatic cancer in vitro
摘要
Abstract
Objective:To observe the feasibility of gene silencing of MAT1 gene by small interference RNA in human pancreatic cancer cells.Methods:BxPC-3 cells were transfected using chemically synthesized double stranded RNA formulated with liposome.Gene expression of MAT1 was assayed by RT-PCR and Western blot respectively.The cell proliferation assay was carded out by counting alive cells after Trypan blue exclusion.The cells invasion ability was determined by Boyden chamber model.Results:The MAT1 mRNA and protein expression of BxPC-3 cells were significantly down-regulated by small interference RNA compared with the control groups.The expression of MAT1 mRNA was reduced by 55.2% and 64.3% in 24 h and 48 h respectively (P＜0.01).The cell proliferation and invasion ability of BxPC-3 cell were significantly inhibited (P＜0.01).Conclusion:The results suggest that gene silencing of MAT1 by siRNA can inhibit the cell proliferation and invasion of BxPC-3 cells,which may be a target in the gene therapy of human pancreatic cancer.关键词
RNA interference/ cell proliferation/ invasion/ pancreatic cancerKey words
RNA interference/ cell proliferation/ invasion/ pancreatic cancer分类
医药卫生引用本文复制引用
Shineng Zhang,Jianping Liu,Fengqin Xu,Yiwen Wang,Wa Zhong,Shizhen Yuan..MAT1-siRNA体外转染抑制胰腺癌的细胞增殖和侵袭能力的研究[J].中德临床肿瘤学杂志（英文版）,2008,7(5):280-282,3.基金项目
Supported by a grant from the Guangdong Science and Technology Commission (No.04009381). （No.04009381）
