中国电子科技2006，Vol.4Issue(3)：281-286,6.
Study on Wusan Granule Anti-tumor Related Target Gene Screened by Cdna Microarray
Study on Wusan Granule Anti-tumor Related Target Gene Screened by Cdna Microarray
摘要
Abstract
To screen Wusan Granule anti-tumor related target gene using cDNA microarray technique, both mRNA from Lewis lung carcinoma tissues treated by Wusan Granule and untreated control are reversibly transcribed to prepare cDNA probes which are labeled by Cy5 and Cy3. Then, the probes are hybridized to the mice cDNA microarray type MGEC-20S. After hybridization, the cDNA microarray is scanned by ScanArray 3 000 scanner and the data is analyzed by ImaGene 3 software to screen the differentially expressed genes. There are 45 differentially expressed genes including 18 known genes and 27 unknown genes between the two groups, and among them, 20 elevated genes and 25 reduced genes are identified. Additionally, the genes related to invasion and metastasis of malignant carcinomas are down-regulated and the genes related to apoptosis are up-regulated. The cDNA microarray technique is a high-throughput approach to screen the Wusan Granule anti-tumor related target genes, which allow us to explore the molecular biological mechanism on a genomic scale.关键词
cDNA microarray/Wusan Granule/Lewis lung carcinoma/differential gene expressionKey words
cDNA microarray/Wusan Granule/Lewis lung carcinoma/differential gene expression分类
数理科学引用本文复制引用
YOU Zi-li,SHI Jin-ping,CHEN Hai-hong..Study on Wusan Granule Anti-tumor Related Target Gene Screened by Cdna Microarray[J].中国电子科技,2006,4(3):281-286,6.基金项目
Supported by the Key Technologies R & D Program of Sichuan Province (No.01SGG0301) （No.01SGG0301）
