江苏大学学报（医学版）2008，Vol.18Issue(4)：281-286,290,7.
整合蛋白β1亚基过表达上调p27抑制肝癌细胞SMMC-7721的增殖
Integrin β1 overexpression inhibits cell proliferation by upregulating p27 in human hepatocellular carcinoma cell line SMMC-7721
摘要
Abstract
Objective: To investigate the mechanism of integrin β1 overexpression regulating cell proliferation of hepatocellular carcinoma cell line SMMC-7721. Methods: MTT and FACS assay were used to analyz the influence of integrin β1 overexpression on cell proliferation and cell cycle. The protein and mRNA expression of p27 were measured by Western blot and RT-PCR. RNAi approach was employed to silence the expression of p27. Then this plasmid(SiRNA-p27) was transfected into integrin β1 overexpressing cells, and cell growth was analyzed. Results: Overexpression of integrin β1 inhibited cell growth in SMMC-7721 cells and induced G1 phase arrest. Integrin β1 overexpression increased the protein level of p27, but not mRNA level. siRNA-p27 plasmid effectively silenced the expression of p27, and knock-down of p27 blocked the cell growth inhibition induced by integrin β1 overexpression. Conclusion: Overexpression of integrin β1 inhibited cell proliferation by upregulating p27 in human hepatocellular carcinoma cell line SMMC-7721.关键词
整合蛋白/过表达/p27/细胞增殖/RNA干扰Key words
integrin/overexpression/p27/cell proliferation/RNA interference分类
生物科学引用本文复制引用
傅奕,梁玉龙..整合蛋白β1亚基过表达上调p27抑制肝癌细胞SMMC-7721的增殖[J].江苏大学学报（医学版）,2008,18(4):281-286,290,7.基金项目
This work was supported by The National Natural Science Foundation of China(30570963). （30570963）
