中国水产科学2005，Vol.12Issue(3)：281-287,7.
条斑紫菜抗坏血酸过氧化物酶基因的cDNA克隆和序列分析
cDNA cloning and sequence characterization of an ascorbate peroxidase from marine red alga Porphyra yezoensis
摘要
Abstract
Screening of a cDNA library from Porphyra yezoensis with probe based upon cytosolic ascorbate peroxidase (APX,EC 1.11.1.11) genes identified a full-length clone apparently encoding a novel type of ascorbate peroxidase.The clone contained an open reading frame for a protein of 242 amino acids,which exhibited sequence similarity to known cytosolic APX from higher plants and contained two motifs conserved in peroxidases.The deduced amino acid sequence of P.yezoensis APX showed a hybrid-type structure,which was only found previously in an APX-B from a unicellular red alga Galdieria partite,similar to both chloroplastic and cytosolic isoforms of higher plants.It was possible that the hybrid-type structure was an important characteristic of red algal APX.APX cDNA from P.yezoensis showed a high (G+C) content (88.4% in the third-base position and 63.7% total) in the coding region.The nucleotide sequence data reported in this paper will appear in the GenBank database under accession number AY282755.关键词
cDNA/抗坏血酸过氧化物酶/条斑紫菜/红藻Key words
cDNA/ascorbate peroxidase/Porphyra yezoensis/Galdieria partite分类
农业科技引用本文复制引用
马凌波,张凤英,马春艳,王素娟..条斑紫菜抗坏血酸过氧化物酶基因的cDNA克隆和序列分析[J].中国水产科学,2005,12(3):281-287,7.基金项目
Chinese Academy of Fishery Sciences Grant(2003-08) （2003-08）
