中国肿瘤生物治疗杂志2000，Vol.7Issue(4)：282-284,3.
突变N-ras基因重组痘苗病毒rV-N-ras/61的构建
Construction of Mutant N-ras/61 Gene Recombinant Vaccinia Virus
摘要
Abstract
Objective: To construct the recombinant vaccinia virus of mutant N-ras/61 gene and enhance the immunogenecity of mutant N-ras／61 protein produced by the recombinant vaccinia virus. Methods: N-ras／61 gene was inserted into P1108 and transfected into CV-1 cell infected with vaccinia virus by Cell FECTIN. PCR and Western blot were used to identify the recombinants. Results: We get recombinant vaccinia virus rV-N-ras/61 by PCR and tk- selecting. The expression of N-ras gene was detected by Western blot. Conclusion: This study is a test for studing effective vaccine of mutant N-ras/61 gene. The efficacy in vivo of the N-ras／61 vaccine in immunotherapy is under investgation.关键词
痘苗病毒/N-ras基因/突变/疫苗Key words
vaccinia virus/N-ras gene/mutant/vaccine分类
生物科学引用本文复制引用
张建,赵跃然,游力,高春义,田志刚..突变N-ras基因重组痘苗病毒rV-N-ras/61的构建[J].中国肿瘤生物治疗杂志,2000,7(4):282-284,3.基金项目
本课题受山东省科委科研基金(951166307)资助 （951166307）
