生物技术通报Issue(2)：28-30,3.
梅毒螺旋体重组抗原酶联免疫吸附试验的建立及应用
Development and Application of an Enzyme-linked Immunoassay
for Serodiagnosis of Syphilis by Recombinant Trepnoma
Palidum Antigens
何君 1杜兰英 2庄辉 2黄策 1周育森1
作者信息
- 1. 军事医学科学院微生物流行病所 北京 100071
- 2. 北京大学基础医学院病原生物学系 北京 100083
- 折叠
摘要
Abstract
Objective；To develop an by Enzyme-linked Immu nosorbent Assay (ELISA) by using specific genetic Treponema pallidum recombinant antigen ( TPN17 & TmpA) and evaluate its potential use in the serodiagnosis of Syphilis. Methods The expressed and purified specific antigen were coated microtiter plates, and esta blished the ELISA. The sensitivity, specificity and positive rates of the assay were determined by detecting standard reference syphilis sera, or the sera from patients and blood donors. In addition, we also investigated the positive rates in different population of the country. Results The sensitivity and specificity of the ELISA were both 100%. The identity of the ELISA and TPHA was significantl y higher(95.7%) than that of the RPR and TPHA(89.1%). The positive rate in the s era of the blood donors was 0.3%～0.69%, and it was much lower in normal sera.关键词
梅毒螺旋体/重组抗原/酶联免疫吸附试验/血清Key words
recombinant antigens/Trepnoma pallidum；Enzy me-linked Immunoassay/serum分类
生物科学引用本文复制引用
何君,杜兰英,庄辉,黄策,周育森..梅毒螺旋体重组抗原酶联免疫吸附试验的建立及应用[J].生物技术通报,2001,(2):28-30,3.
