现代妇产科进展2001，Vol.10Issue(1)：28-30,3.
子宫颈鳞状细胞癌细胞外间质胶原重构及其调控机制的研究
Study on collagen remod eling in t he extracellular matrix of cervical carcinoma and the related regulating mechani sm s.
娄阁 1高庆玉 1马玉彦 1隋丽华 1马宝璋1
作者信息
摘要
Abstract
Objective:To explore the mechanisms of extracellular matri x rem odeli ng in the cervical squamous cell carcinoma.Methods:The single an d dual fluorescent labeling techniques and confocal laser scanning microscopy we re used to demonstrating the expression of type Ⅰ,Ⅲ and Ⅳ collagen and relate d matrix metalloproteinases(MMP-1 and MMP-2),membrane-type metalloproteinases (M T1-MMP) and its tissue inhibitor (TIMP-2) in formalin-fixed,paraffin embedded no rmal cervical and squamous cell carcinoma tissue.Results:The sub epithelial and vessel basement membrane staining of collagen Ⅳ revealed stronge r staining in normal cervical tissue,and the tense expression of type Ⅰ and Ⅲ collagen appeared in the ECM.Immunore-activity(IR) for MMP-1,MMP-2,MT1-MMP and TIMP-2 was very weak.In the cervical squamous cell carcinoma cases,three types c o llagen expression was decreased.The structure of type Ⅰ and Ⅲ collagen was sca tter,and the staining for collagen Ⅳ showed discontinuity,frag mentation or absence of basement membrane in the vicinity of cervical carcinoma, particularly in low-grade invasive carcinomas.The IR for MMP-1,MMP-2,MT1-MMP and TIMP-2 was stronger than that of normal control tissue.The expression of MMP-1, MMP-2 and MT1-MMP was more distinct in the low-grade of differentiation of carci nomas,but the TIMP-2 expression was reverse. Conclusions:In the cervical sq uamous cell carcinoma cases,the type Ⅰ,Ⅲ and Ⅳ collagen was degraded,and the ECM was remodeling,which was correlated to grade of differentiation.The expressi ons of MMP-1,MMP-2,MT1-MMP,and TIMP-2 proteins take part in the mechanisms of EC M remodeling.关键词
宫颈肿瘤/胶原/金属蛋白酶类分类
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娄阁,高庆玉,马玉彦,隋丽华,马宝璋..子宫颈鳞状细胞癌细胞外间质胶原重构及其调控机制的研究[J].现代妇产科进展,2001,10(1):28-30,3.
