中国人兽共患病杂志2001，Vol.17Issue(1)：28-30,44,4.
套式PCR和DNA探针技术检测Q热立克次体
DETECTION OF COXIELLA BURNETTI BY NESTED PCR AND DNA PROBE
摘要
Abstract
Aim To establish the rapid and sensitive methods for detection ofCoxiella burnetti (Cb).Methods The nested PCR was established by using two sets of primers designed based on the intervening sequence(IVS) in 23S rRNA gene of Cb and the amplimer of Cb genomic DNA was labeled with 32P as a probe in dot-blot hybridization.Results The positive results were observed in all 9 Cb strains but not in controls in both NPCR and hybridization assays. The nested PCR and IVS probe detected as little as 0.1pg and 0.5ng Cb genomic DNA, respectively. The blood of guinea-pigs, the blood and spleens of mice infected with Cb were positive in both assays. Conclusion The nested PCR and IVS probe are highly sensitive and specific for detection of Cb and they may be more useful in diagnosis of Q fever.关键词
套式PCR/DNA探针/Q热立克次体分类
医药卫生引用本文复制引用
陈荣,温博海,张雪,俞树荣..套式PCR和DNA探针技术检测Q热立克次体[J].中国人兽共患病杂志,2001,17(1):28-30,44,4.基金项目
国家自然科学基金资助课题(批准号:39740008) （批准号:39740008）
