河南医科大学学报2000，Vol.35Issue(4)：283-285,3.
蝎毒抗癌多肽组分Ⅲ的分离方法研究
Isolation and purification of antineoplastic polypeptide-Ⅲ from APBMV
摘要
Abstract
Aim: To explore the methods on isolation and purification of antineoplastic polypeptide-Ⅲ from APBMV (AP-Ⅲ ). Methods: The AP-Ⅲ was isolated from scorpion venom of Buthus martensii karsch by pretreatment, ion-exchange chro-matography (Sepharose FF, column:25 cm × 5 cm, flow rate:0. 8 ml/min, linear gradient: 1 200 min) or coupling gel filtration(Sephadex G-50, column: 100 cm× 5 cm,flow rate:0.5 ml/min) with Sepharose FF chromatography. The purity of AP-Ⅲ andother compositions of scorpion venom were inspected by high performance liquid chromatography. Results: The purification rateand output of AP- Ⅲ were 89% and 2.24 % with ion-exchange chromatography only, but 95 % and 4.72 % with coupling SephadexG-50 gel filtration with Sepharose FF chromatography. Conclusion: The fractionating method of coupling Sephadex G-50 gel filtra-tion with Sepharose FF chromatography is useful for improving the purification rate and output of AP- Ⅲ. The experimental result isthought as an important evidence to make clinical use of AP- Ⅲ in treatment of tumor.关键词
蝎毒/分离/纯化Key words
scorpion venom/isolation/purification分类
医药卫生引用本文复制引用
孔天翰,郭进武,肖桂元,杨卓平,周少雄,韩雪飞,陈华艳,董伟华..蝎毒抗癌多肽组分Ⅲ的分离方法研究[J].河南医科大学学报,2000,35(4):283-285,3.基金项目
河南省“九五”科技攻关项目 9512001 （）
