中国中西医结合杂志2001，Vol.21Issue(4)：283-285,3.
热毒清对HL-60细胞产生分泌型肿瘤坏死因子α及
肿瘤坏死因子α转换酶mRNA表达的影响
Effect of Reduqing on HL-60 Cells in Secreting Tumor Necrosis Factor α Production and on TNFα Converting Enzyme mRNA Expression
王开富 1李鸣真 1杨渝珍 2韩玲 2丁廷波 2陆付耳1
作者信息
- 1. 华中科技大学同济医学院附属同济医院中西医结合研究所,
- 2. 华中科技大学同济医学院实验医学中心分子生物室
- 折叠
摘要
Abstract
To explore the molecular mechanism of inhibiting effects of Reduqing on the release of tumor necrosis factorα (TNFα). Methods: Using cytobiologic and molecular biologic technique to observe the effects of Reduqing on HL-60 cells in producing inflammatory cytokine secreting TNFα (sTNFα) and on mRNA expression of TNFα converting enzyme (TACE). Results: (1) Reduqing, diluted in ratio 1∶30, could effectively inhibit the increased HL-60 production of sTNF-α induced by lipopolysaccharides (LPS); (2) Although no obvious effect on TACE was shown when Reduqing was applied alone on HL-60 cells, there was evident inhibitory effect of Reduqing on TACE mRNA expression enhancement induced by LPS. Conclusion: Reduqing could have the double inhibitory effects both on sTNF-α production and on the gene expression of its key enzyme, i.e. TACE stimulated by LPS, suggesting that it might be a hopeful and excellent natural TACE inhibitor.关键词
热毒清 HL-60细胞 肿瘤坏死因子α转换酶 基因表达分类
医药卫生引用本文复制引用
王开富,李鸣真,杨渝珍,韩玲,丁廷波,陆付耳..热毒清对HL-60细胞产生分泌型肿瘤坏死因子α及
肿瘤坏死因子α转换酶mRNA表达的影响[J].中国中西医结合杂志,2001,21(4):283-285,3.
