中国海洋湖沼学报（英文版）2009，Vol.27Issue(2)：283-287,5.DOI:10.1007/s00343-008-0135-z
The preparation and antioxidant activity of glucosamine sulfate
The preparation and antioxidant activity of glucosamine sulfate
摘要
Abstract
Glucosamine sulfate was prepared from glucosamine hydrochloride that was produced by acidic hydrolysis of chitin by ion-exchange method. Optical rotation and elemental analysis characterized the degree of its purity. In addition, the antioxidant potency of chitosan derivative-glucosamine sulfate was investigated in various established in vitro systems, such as superoxide (O(2))/hydroxyl (·OH) radicals scavenging, reducing power, iron ion chelating. The following results are obtained: first, glucosamine sulfate had pronounced scavenging effect on superoxide radical. For example the O(2) scavenging activity of glucosamine sulfate was 92.11% at 0.8 mg/mL. Second, the ·OH scavenging activity of glucosamine sulfate was also strong, and was about 50% at 3.2 mg/mL Third, the reducing power of glucosamine sulfate was more pronounced. The reducing power of glucosamine sulfate was 0.643 at 0.75 mg/mL.However, its potency for ferrous ion chelating was weak. Furthermore, except for ferrous ion chelating potency, the scavenging rate of radical and reducing power of glucosamine sulfate were concentration-dependent and increased with their increasing concentrations, but its ferrous ion chelating potency decreased with the increasing concentration. The multiple antioxidant activities of glucosamine sulfate were evidents of reducing power and superoxide/hydroxyl radicals scavenging ability. These in vitro results suggest the possibility that glucosamine sulfate could be used effectively as an ingredient in health or functional food, to alleviate oxidative stress.关键词
glucosamine sulfate/ ion-exchange method/ radical scavenging effect/ reducing power/chelating effectKey words
glucosamine sulfate/ ion-exchange method/ radical scavenging effect/ reducing power/chelating effect分类
地球科学引用本文复制引用
XING Ronge,LIU Song,WANG Lin,CAI Shengbao,YU Huahua,FENG Jinhua,LI Pengcheng..The preparation and antioxidant activity of glucosamine sulfate[J].中国海洋湖沼学报（英文版）,2009,27(2):283-287,5.基金项目
Supported by the Talent Program of Chinese Academy of Sciences(Dongbei Zhi Chun) and Award Foundation of Scientific Research for Excellent Young and Middle-Age Scientist of Shaedong Province (No.2007BS07002) （Dongbei Zhi Chun）
