林业科学2007，Vol.43Issue(1)：28-34,插2,8.
细胞分裂素和硝酸铵对欧洲云杉不定芽伸长的影响
Influence of Cytokinin and Ammonium Nitrate on Elongation of Adventitious Buds in Norway Spruce( Picea abies )
Dietrich Ewald 1胡建军2
作者信息
- 1. 德国联邦林业及林产品研究中心林木遗传育种研究所,Waldsieversdorf,15377
- 2. 中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京,100091
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摘要
Abstract
The capability of different cytokinins (BA, kinetin, zeatin, 2iP) to stimulate the induction and development of adventitious buds was determined after single and repeated induction steps with hypocotyl explants. BA was the most effective cytokinin as far as it concerned the number of induced adventitious buds. Zeatin, although less effective for this pattern,promoted the elongation of buds even after repeated induction cycles contrary to BA. This is important to get propagating bud clusters with the ability to form elongating shoots. A five-year-old propagating clone line of adventitious bud clusters was used to study the influence of zeatin and ammonium nitrate on the shoot elongation. Three induction variants (6.84, 13.68,20.52 μmol· L-1 zeatin, each with 0.69 μmol· L-1 kinetin for 4 weeks) were tested. The highest zeatin concentration induced the largest number of elongating buds. The influence of one subculture step with an enhanced ammonium nitrate concentration during the growth regulator free shoot development period (4 subcultures) was tested. A subculture on a medium free of growth regulators with a low ammonium nitrate concentration (2.5 mmol· L-1) followed the cytokinin containing induction medium. The next subculture medium was supplemented with 2.5, 7.5, 12.5 and 17.5 mmol· L-1 ammonium nitrate. An 8-week period (2subcultures) followed with 2.5 mmol· L-1 ammonium nitrate. The treatment with 7.5 mmol· L-1 ammonium nitrate formed the highest number of elongating buds. This was identical for all zeatin concentrations used for induction. Increased ammonium nitrate concentrations during this treatment (12.5 mmol· L-1 and 17.5 mmol·L-1) decreased the number of elongating buds.关键词
欧洲云杉/不定芽/细胞分裂素/硝酸铵Key words
Norway Spruce ( Picea abies)/adventitious buds/cytokinin/ammonium nitrate分类
农业科技引用本文复制引用
Dietrich Ewald,胡建军..细胞分裂素和硝酸铵对欧洲云杉不定芽伸长的影响[J].林业科学,2007,43(1):28-34,插2,8.
