南京医科大学学报(英文版)2009，Vol.23Issue(4)：284-288,5.
Chrysanthemum indicum linné extracts attenuate the mitogenic effect of epinephrine on human HCC cells
Chrysanthemum indicum linné extracts attenuate the mitogenic effect of epinephrine on human HCC cells
Aihua Yuan 1Zongfang Li 2Xinqiu Li 1Shanyong Yi 3Shukui Wang 4Kaiwang Shi 2Jianmin Bian2
作者信息
- 1. Department of General Surgery, Second Affiliated Hospital of Medical College of Xi' an Jiaotong University, Xi' an, China
- 2. Department of General Surgery, Nanfing First Hospital Affiliated to Nanfing Medical University, Nanjing, China
- 3. Department of Hepatobiliary Surgery, First Affiliated Hospital of Medical College of Xi' an Jiaotong University, Xi' an, China
- 4. Department of Cancer Center, First Affiliated Hospital of Medical College of Xi' an Jiaotong University, Xi' an, China
- 折叠
摘要
Abstract
Objective: To investigate the stimulatory effect of epinephrinetEpi) and the antagonistic effect of Chrysanthemum indicum Linné extracts (CILE) on Epi-induced growth of human hepatocellular carcinoma(HCC) cells. Methods: The stimulatory effect of Epi and inhibitory effect of CILE on the growth of HepG2 and MHCC97H cells were investigated using a proliferation assay in correlation with β adrenergic receptor(β 2-AR) blockade, a MEK1/MEK2 inhibitor, and assessment of MAPK/ERKI/2 intracellular activity. Results: Epi transiently activated MAPK/ERK 1/2 in HepG2 and MHCC97H cells, resulting in a burst of growth. The effect of Epi was significantly attenuated by ICI 118551 and U0126. CILE exhibited a dose-dependent attenuation of the stimulatory effect of Epi on the growth of both cell lines and inhibited the Epi-induced activation of MAPK/ERK1/2. Conclusion: Epi, mimicking a mitogen, stimulated the growth of HepG2 and MHCC97H cells, and CILE was effective in attenuating this effect of Epi on tamor cells by inhibiting the β 2-AR-mediated activation of MAPK/ERK1/2.关键词
Chrysanthemum indicum Linn6 extracts/epinephrine/extracellular signal-regulated kinase 1/2/hepatocellular carcinomaKey words
Chrysanthemum indicum Linn6 extracts/epinephrine/extracellular signal-regulated kinase 1/2/hepatocellular carcinoma分类
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Aihua Yuan,Zongfang Li,Xinqiu Li,Shanyong Yi,Shukui Wang,Kaiwang Shi,Jianmin Bian..Chrysanthemum indicum linné extracts attenuate the mitogenic effect of epinephrine on human HCC cells[J].南京医科大学学报(英文版),2009,23(4):284-288,5.
