四川大学学报（自然科学版）2007，Vol.44Issue(2)：284-288,5.
剪切力环境下的血管平滑肌细胞图像分割技术研究
Cell segmentation of vascular smooth muscle cells under shear stress using image processing
摘要
Abstract
Quantitatively analysis of the morphological response to shear stress on vascular smooth muscle cells (VSMCs) will facilitate understanding the growth mechanism and pathology of VSMCs. Image segmentation plays a key role in image analysis and is also the most difficult step in image processing pipeline. In this paper,firstly, the characteristics of the images of VSMCs cultured in the parallel plate chamber is analyzed in detail,then one segmentation strategy which has four steps based on watershed operators is proposed. Image preprocessing, illumination correcting, mathematical morphological reconstruction, watershed transformation are implemented according to the set sequence. The result shows a better separation cells from background can be achieved by using this method. At the end the result of image segmentation is analyzed and discussed.关键词
定量分析/分水岭转换/数学形态学/图像分割Key words
quantitatively analysis/watershed/mathematical morphology/image segmentation分类
信息技术与安全科学引用本文复制引用
李晓宁,樊瑜波..剪切力环境下的血管平滑肌细胞图像分割技术研究[J].四川大学学报（自然科学版）,2007,44(2):284-288,5.基金项目
国家自然科学基金(10527001,10672105) （10527001,10672105）
北京R&D项目(H060920051030) （H060920051030）
四川师范大学基金 （）
