河南医科大学学报2000，Vol.35Issue(4)：285-288,4.
蝎毒抗癌多肽与其组分Ⅲ的毒性比较
Comparison of the toxicity of APBMV and its composition AP-Ⅲ
摘要
Abstract
Aim:In order to apply antineoplastic polypeptide from Buthus Martensii venom (APBMV) and antineoplasticpolyptide-Ⅲ from APBMV(AP-Ⅲ ) to clinical treatment at maximum safe degree, and to evaluate the toxicosis medicinalis andside-effect. Methods:120 mice(20 ～ 22 g) were divided at random into 12 groups( 10 mice/group), and injected by ip withAPBMV(Group1 ～ 6) or AP-Ⅲ (Group7 ～ 12) in 0.2 ml/20 g of different concentrations. The conduct and pathological changesin organs of animals were observed after drug injected. The LD50 and L95 of APBMV or AP- Ⅲ were evaluated with the method ofcomplex count. Results: 1 ～ 5 min after injecting APBMV or AP- Ⅲ in higher concentration, the changes of animal behaviourswere found from screaming, jumping, lapping the injected position, slobbering to irregular breath. With the prolongation of sur-vival time, the animals were quiescent, spastic paralystic, breathed quickly, stifled, and were convulsive until dead. Most ani-mals were dead in 90 min after injection and then anatomized. The pathological change was not found by naked eye in importantinternal organs of dead animals. According to the drug and dead rate, the LD50 of APBMV was 9. 738 mg/kg and L95 was from8.211 mg/kg to 11.265 mg/kg,the LD50 of AP-Ⅲ was 8.17 mg/kg and L95 was from 6.898 mg/kg to 9.456 mg/kg. Conclu-sion: The LD50 of AP- Ⅲ is lower than APBMV' s, which means the acute toxicity of the former is higher than that of the latter.关键词
蝎毒/抗癌/多肽/LD5oKey words
scorpion venom/antineoplastic/polypeptide/LD50分类
医药卫生引用本文复制引用
董伟华,郭进武..蝎毒抗癌多肽与其组分Ⅲ的毒性比较[J].河南医科大学学报,2000,35(4):285-288,4.基金项目
河南省“九五”科技攻关项目 951200102 （）
