内科理论与实践2009，Vol.4Issue(4)：285-291,7.
外体负载特异性抗原促进小鼠抗肿瘤效应的研究
Effect of exosomes pulsed with specific tumor antigen in stimulating cytotoxic T-lymphocyte responses and antitumour immunity in mice
摘要
Abstract
Objective To validate the human dendritic cell(DC) derived-exosomes(EXO) loaded with NY-ESO-1 effects of antigen in promoting the amplification of specific cytotoxic T lymphocytes (CTLs)in transgenic mice and enhancing the anti-tumor effect. Methods Peripheral blood mononuclear cells (PBMC) obtained from human leukocyte antigen (HLA)-A2-positive healthy adults were used to induce and isolate mature DC. A series of differential ultracentfifugation combined with ultrafiltration were used to isolate the EXO secreted by DC from the supematant. Then the EXO was loaded with testis cancer antigen NY-ESO-1 (EXO/Ag), and used in conjunction with polyinosinic acid-polycytidylic acid(Polyl-C) to immunize healthy HLA-A2 transgenic C57 mice. The spleen cells were cultured in vitro and the EXO/Ag was added to the system. Spleen cells were tested for tetranser staining and eytotoxicity against K562 cell and melanoma cell(control) killed. Results NY-ESO-1-specific CTLs were amplified and displayed cytotoxic effect on tumor cell line K562. In vitro study showed that NY-ESO-1-specific CTLs amplified substantially, and their killing effect on tumor cell line K562 was greatly enhanced. Conclusions EXO/Ag in conjunction with PoIyI-C has the prospect to be a new generation of anti-tumor vaccine.关键词
肿瘤疫苗/外体/NY-ESO-1抗原肽/聚肌胞/佐剂/肿瘤免疫/Toll样受体/人类白细胞抗原-A2转基因小鼠Key words
Tumor vaccine/Exosomes/NY-ESO-1 antigenic peptide/Polyinosinic acid-polycytidylic acid/Adjuvant/Tumor immunity/Toll-like receptor/, Human leukocyte antigen-A2 transgenic mice分类
医药卫生引用本文复制引用
任微娜,常春康,范华骅..外体负载特异性抗原促进小鼠抗肿瘤效应的研究[J].内科理论与实践,2009,4(4):285-291,7.基金项目
上海市科委课题经费资助(项目编号:0552nm010) （项目编号:0552nm010）
