医学分子生物学杂志2009，Vol.6Issue(4)：287-291,5.DOI:10.3870/j.issn.1672-8009.2009.04.002
5-氟尿嘧啶增强HeLa细胞对自然杀伤细胞敏感性研究
Enhancement of Susceptibility of HeLa Cells to NK Lysitic Activity through Upregu lation of NKG2D Ligand MICA by 5-FU Treatment
摘要
Abstract
Objective To investigate the expression of NKG2D ligand MICA in HeLa cells treated by 5 - fluorouracil (5-FU) and the susceptibility of treated HeLa cells to NK92 cell-mediated killing.Methods HeLa cells were treated with different concentrations of 5-FU. Expression of MICA in RNA and protein level was detected by semi-quantitative PCR and flow cytometry. NK cell-mediated killing of HeLa cells was determined by MTT assay. The cytolytic activity of NK92 cells was compared in the presence or absence of NKG2D antibody.Results The results of semi-quantitative RT-PCR showed that MICA was expressed with the increase of 5-FU dose range and upregulated along with the exposure time course (0h, 8h, 16h, 24h) of HeLa cells to 5-FU. Flow cytometry data demonstrated that the upregulation of MICA expression mainly result from the non-apoptotic cells. The cytolytic effect of NK92 cells on HeLa cells was enhanced by 5-FU treatment for 24h at different effect/target ratio. NK-cell mediated killing of HeLa cells was partially inhibited by NKG2D antibody.Conclusion 5-FU increases cell surface expression of NKG2D ligand MICA on HeLa cells, thus enhancing the sensitivity of Hela cells to lysitic effect of NK92 cells, suggesting that combination of chemotherapy and immunotherapy with NK cells might improve the treatment of cervical cancer patients.关键词
5-氟尿嘧啶/NKG2D配体/主要组织相容性复合物Ⅰ类链相关分子A/HeLa细胞/NK92细胞Key words
5-fluorouracil/natural killer cell group 2D/ligands/MICA/HeLa cells/NK92 cells分类
医药卫生引用本文复制引用
张妍,魏广智,赵敬湘,罗丹,王字玲..5-氟尿嘧啶增强HeLa细胞对自然杀伤细胞敏感性研究[J].医学分子生物学杂志,2009,6(4):287-291,5.基金项目
军事医学科学院创新基金重大项目(No.2008ZD011)This work was supported by a grant from the Academy of Military Medical Sciences (No. 2008ZD011) （No.2008ZD011）
